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smart seq mrna kit  (TaKaRa)


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    Structured Review

    TaKaRa smart seq mrna kit
    Smart Seq Mrna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+mrna+kit/SMART-Seq+mRNA/pm42883050-72-26-29
    Average 99 stars, based on 171 article reviews
    smart seq mrna kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    other:

    Article Title: Maternal granulocyte colony-stimulating factor alters synaptic maturation and social behaviors in offspring.
    Article Snippet: 14 Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Hilden, Germany), and RNA integrity was assessed with the Agilent 4200 TapeStation System (Agilent Technologies, CA, USA). cDNA libraries were prepared with the SMART-Seq mRNA kit (Takara Bio, Shiga, Japan) and sequenced on the NovaSeq 6000 platform (Illumina, San Diego, CA, USA) to generate 150 bp paired-end reads.

    Article Title: Apolipoprotein E Deficiency Impairs Human Microglial Proliferation Accompanied by Elevated Cellular Oxidative Stress.
    Article Snippet: Total RNA was extracted using the miRNeasy Mini Kit (Qiagen).

    Article Title: Apolipoprotein E Deficiency Impairs Human Microglial Proliferation Accompanied by Elevated Cellular Oxidative Stress
    Article Snippet: Total RNA was extracted using the miRNeasy Mini Kit (Qiagen).

    Synthesized:

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules.
    Article Snippet: Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN) following the manufacturer’s protocol, and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Subsequently, the double-stranded cDNA was fragmented and tagged at both ends through a tagmentation reaction using the Nextera XT DNA Library Prep Kit (Illumina Inc.), which applies transposon-mediated fragmentation and adapter insertion.

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules
    Article Snippet: % inhibition = ( 1 − ( Sample − LowControl ) / ( HighControl − LowControl ) ) ∗ 100 pIC50 values are calculated by curve fitting using a four parameter logistic equation using XLfit (IDBS). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Small interfering RNAs (siRNAs) targeting human TNIK (ID: E−004542-00) and a non-targeting scrambled control siRNA (D-001910-10) were purchased from Dharmacon.

    Article Title: Condensate corona-nanoparticle complexes transfer functional biomolecules between cells.
    Article Snippet: To minimize dilution, 20 μl of the first eluate was reapplied for a second elution, and mRNA was stored at −80 °C. mRNA integrity and size distribution were verified with a bioanalyser on the day of extraction (or the following day) using the RNA 6000 Pico and Small RNA kits (Agilent, 5067-1548). .. Nature Materials Article https://doi.org/10.1038/s41563-026-02534-5 Generation and amplification of total cDNA Full-length cDNA was synthesized from 100 ng of total RNA using the SMART-Seq mRNA kit (Takara Bio) under RNase-free conditions (RNaseZap decontamination; Invitrogen, AM9780). ..

    Polymerase Chain Reaction:

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules.
    Article Snippet: Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN) following the manufacturer’s protocol, and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Subsequently, the double-stranded cDNA was fragmented and tagged at both ends through a tagmentation reaction using the Nextera XT DNA Library Prep Kit (Illumina Inc.), which applies transposon-mediated fragmentation and adapter insertion.

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules
    Article Snippet: % inhibition = ( 1 − ( Sample − LowControl ) / ( HighControl − LowControl ) ) ∗ 100 pIC50 values are calculated by curve fitting using a four parameter logistic equation using XLfit (IDBS). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Small interfering RNAs (siRNAs) targeting human TNIK (ID: E−004542-00) and a non-targeting scrambled control siRNA (D-001910-10) were purchased from Dharmacon.

    Amplification:

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules.
    Article Snippet: Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN) following the manufacturer’s protocol, and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Subsequently, the double-stranded cDNA was fragmented and tagged at both ends through a tagmentation reaction using the Nextera XT DNA Library Prep Kit (Illumina Inc.), which applies transposon-mediated fragmentation and adapter insertion.

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules
    Article Snippet: % inhibition = ( 1 − ( Sample − LowControl ) / ( HighControl − LowControl ) ) ∗ 100 pIC50 values are calculated by curve fitting using a four parameter logistic equation using XLfit (IDBS). .. Employing the Switching Mechanism at 5′ End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. .. Small interfering RNAs (siRNAs) targeting human TNIK (ID: E−004542-00) and a non-targeting scrambled control siRNA (D-001910-10) were purchased from Dharmacon.

    Article Title: Condensate corona-nanoparticle complexes transfer functional biomolecules between cells.
    Article Snippet: To minimize dilution, 20 μl of the first eluate was reapplied for a second elution, and mRNA was stored at −80 °C. mRNA integrity and size distribution were verified with a bioanalyser on the day of extraction (or the following day) using the RNA 6000 Pico and Small RNA kits (Agilent, 5067-1548). .. Nature Materials Article https://doi.org/10.1038/s41563-026-02534-5 Generation and amplification of total cDNA Full-length cDNA was synthesized from 100 ng of total RNA using the SMART-Seq mRNA kit (Takara Bio) under RNase-free conditions (RNaseZap decontamination; Invitrogen, AM9780). ..

    Generated:

    Article Title: Integration of transcriptional signatures from brain tissue and plasma extracellular vesicles of a preclinical tauopathy mouse model
    Article Snippet: Immunoreactive signals were visualized using enhanced chemiluminescence (TMA-6, Lumigen ECL Ultra, MI) with the Amersham Imager 600 (GE Lifescience). .. mRNA libraries were generated using the SMART-Seq mRNA Kit (#634773, Takara, CA) combined with the Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina, CA) and Illumina DNA/RNA UD Indexes Set A (#20091654, Illumina, CA). .. Small ncRNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set (#E7560S, NEB, MA).

    DNA Library Preparation:

    Article Title: Integration of transcriptional signatures from brain tissue and plasma extracellular vesicles of a preclinical tauopathy mouse model
    Article Snippet: Immunoreactive signals were visualized using enhanced chemiluminescence (TMA-6, Lumigen ECL Ultra, MI) with the Amersham Imager 600 (GE Lifescience). .. mRNA libraries were generated using the SMART-Seq mRNA Kit (#634773, Takara, CA) combined with the Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina, CA) and Illumina DNA/RNA UD Indexes Set A (#20091654, Illumina, CA). .. Small ncRNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set (#E7560S, NEB, MA).

    Lysis:

    Article Title: Single-Cell RNA Sequencing Reveals Molecular Differences Underlying Meiotic Competence of Brilliant Cresyl Blue (BCB) Selected Alpaca Oocytes.
    Article Snippet: .. Finally, each oocyte was individually transferred in a final volume of 1 μL into a 0.2-mL tube containing 9.5 μL of lysis buffer provided in the SMART-Seq mRNA Kit (Takara, Shiga, Japan). ..



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